Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.
نویسندگان
چکیده
Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).
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عنوان ژورنال:
- Nucleic acids research
دوره 3 2 شماره
صفحات -
تاریخ انتشار 1976